571 Dendritic cell programming in vaccinia skin scarification

Smallpox is one of the deadliest viral diseases in human history, killing an estimated 300 million since 1900 alone. Edward Jenner’s 1796 discovery that skin inoculation with cowpox a scarification method delivery[i][ii] induced immunity against smallpox led to global eradication disease by 1980[iii]. In recent years, vaccinia virus model derived from was used demonstrate (VACVss) superior immunization strategy other methods vaccine delivery[iv]. Relative intradermal or intramuscular injection (i.m.), generates optimal adaptive immunity, indicated VACV-specific CD8+ T memory and IgG titers[v]. The cellular mechanisms behind requirement for have not been elucidated. To test how confers protective we wanted Dendritic Cell (DC) directed modified scarification, focusing on Batf3-dependent[vi] CD103+ dermal DC1s expression DNGR1 death receptor cross-present antigens achieve successful immunity[vii],[viii],[ix],[x],[xi]. However, it unknown contributes responses licensing. We generated RNA microarray dataset order examine transcriptomic changes DC1 cells response (VACV SS)[xii]. Transcriptome analysis revealed migratory (CD103+ DCs) distinct gene modules responding VACVss, supporting candidate pathways interrogate productive licensing cues.